Research Technician II

Employment Type
Regular Full-Time
Requisition number
1700
Department
Harrison Lab
Location : City
Seattle

Overview

This position is responsible for providing technical laboratory support for biomedical research projects performing both routine and non-routine lab procedures. The Harrison Lab works to understand the mechanisms controlling host-microbe interactions at barrier sites such as the skin and gastrointestinal tract.

We study how our resident commensal microbes influence the development, education and function of our immune system. To do so, we utilize a multi-disciplinary approach combining development and implementation of tools to track commensal-specific T and B cells in healthy and inflamed tissues, with single-cell and population level transcriptomics and epigenetic analyses of T and B cell differentiation to investigate how the host mounts and regulates immunity to the microbiome.

Responsibilities

  • Preparing laboratory reagents, chemicals, stocks, cultures and cell lines.
  • Basic molecular biology (cloning, plasmid isolation and transformation).
  • Production and purification of proteins
  • Working with mice, including immunization and surgeries if this is the focus of the project.
  • Preparing samples from human blood, stool and tissue using sterile technique.
  • Maintain laboratory records by recording detailed data, including photography and computer input, in a literate and thorough manner.
  • Conduct lab inventory, complete purchase orders for hardware, glassware, and supplies.

Requirements

  • Requires a BA/BS in a scientific field and a minimum of 2-3 years of progressively responsible work experience in a scientific research laboratory.
  • Must have strong mathematical, computer, data analysis, and statistical analysis skills.
  • Will also need knowledge of proper operation of complex laboratory instruments and equipment.
  • Must have good verbal and writing skills, and have the ability to work both independently and in a team environment.
  • May require skill in animal surgery techniques.
  • Successful completion and documentation of experiments involving mammalian tissue culture and multiparameter flow cytometry required.

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, citizenship, disability or protected veteran status. 

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